کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2821660 | 1160978 | 2006 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori](/preview/png/2821660.png)
Antimicrobial peptides (AMPs) are a group of immune proteins that protect the host from infection. In Drosophila, seven groups of inducible AMPs have been identified, with activities against fungi and gram-positive and gram-negative bacteria. On the basis of the silkworm genome sequence and expressed sequence tags, we identified 35 AMP genes, mostly belonging to the cecropin, moricin, and gloverin gene families. We predicted the core promoters required for gene transcription and the cis-regulatory elements for NF-κB/Rel and GATA transcription factors. The expression profiles of these genes after an immune challenge with lipopolysaccharide were examined by reverse transcription PCR. Members of the cecropin B and gloverin A subfamilies were intensely expressed in the fat body after induction. In contrast, those of the moricin B subfamily were not expressed under the same conditions. Such results suggest that these regulatory elements and their positions in the upstream regions play an important role in regulating the transcription of these defense genes.
Journal: Genomics - Volume 87, Issue 3, March 2006, Pages 356–365