کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2823845 | 1161437 | 2013 | 6 صفحه PDF | دانلود رایگان |
BackgroundA major problem in the treatment of multiple sclerosis (MS) patients with interferon-beta (IFN-β) is the development of neutralising antibodies (NAbs). High levels of NAbs block the induction of IFN-β-inducible markers, including Myxovirus Resistance Protein A (encoded by the MX1 gene), resulting in a loss of bioactivity and therapeutic benefit.ObjectiveThe primary objective of this study is to investigate the in vivo biological response to interferon-alpha (IFN-α) in MS patients, who have developed neutralising antibodies (NAbs) against IFN-β.Design/MethodsThe study was an open-label phase II study in 10 patients with relapsing-remitting MS with persisting NAbs against IFN-β and absent in vivo mRNA MxA response. We used in vivo induction of MX1 mRNA and other IFN-inducible genes as measure of the biological response. The primary endpoint was the in vivo mRNA MX1 response after an injection of IFN-α compared with the response after an injection of IFN-β.ResultsIn all 10 patients we found high MX1 expression after injection of IFN-α 6 MIU, indicating a preserved in vivo response to IFN-α. We measured the gene expression index of immune system genes in blood cells from the 10 NAb-positive patients after IFN-α treatment and 10 NAb-negative patients after injection of IFN-β. We found a significantly increased expression of a number of genes, including MX1, IFI27, IL10 and TNFSF10 (encoding TRAIL) in the NAb-positive patients treated with IFN-α.InterpretationIFN-α could be a therapeutic option in patients, who have lost the biological response to IFN-β because of NAbs against IFN-β. ClinicalTrials gov. Identifier: NCT01171209.
► IFN-α has a full in vivo biological response in MS patients with NAbs against IFN-β.
► We found a strong induction of MX1 mRNA and of other type I IFN-regulated genes.
► IFN-α could be a therapeutic option in patients with loss of bioactivity to IFN-β.
Journal: Multiple Sclerosis and Related Disorders - Volume 2, Issue 2, April 2013, Pages 141–146