کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
28902 44104 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A chemiluminescence study on degradation of gelatine: Biodegradation by bacteria and fungi isolated from cinematographic films
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
A chemiluminescence study on degradation of gelatine: Biodegradation by bacteria and fungi isolated from cinematographic films
چکیده انگلیسی

Chemiluminescence (CL) has become a sensitive tool for the study of polymer degradation, induced by exposure to various factors, such us heat, UV-light and oxygen. In this paper, the results obtained with this technique in the study of gelatine samples hydrolytically degraded under sterilisation conditions are presented. Also, photographic gelatine exposed to bacterial and fungal degradations, in water solution and under controlled conditions, have been study by the chemiluminescence emission of their corresponding films and the biodegradation extent was determined by viscosity. The bacteria and fungi employed in this work have been isolated from cinematographic films in a previous work.The high intensities of chemiluminescence emission obtained for gelatines biodegraded by bacteria and fungi, in aqueous solution at 37 and 25 °C, respectively, are different to those obtained in the thermal degradation. The hydrolytic degradation mechanism is through a cleavage of the peptide bond of the protein without significant oxidation of the material. In contrast, biodegradation by bacteria and fungi at low temperatures decreases the molecular weight of the gelatine (viscosity) by the enzymatic activity but, also, produces an important oxidation in the material due to the reactive oxygen species (ROS) generated in the microbial metabolism.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 185, Issues 2–3, 25 January 2007, Pages 188–197
نویسندگان
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