|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|29385||44380||2016||6 صفحه PDF||سفارش دهید||دانلود رایگان|
• White light enhanced callus induction frequency in Stevia rebaudiana.
• White light displayed maximum biomass accumulation in log phases.
• Blue lights enhanced the production of phenolics and flavonoids content.
• Blue lights improved total antioxidant capacity.
• Green and red lights promoted reducing power assay and DPPH activity.
Stevia rebaudiana (S. rebaudiana) is a very important species with worldwide medicinal and commercial uses. Light is one of the major elicitors that fluctuate morphogenic potential and biochemical responses. In the present study, we investigated the effect of various spectral lights on biomass accumulation and secondary metabolite production in callus cultures of S. rebaudiana. Leaf explants were placed on Murashige and Skoog (MS) medium and exposed to various spectral lights. 6-Benzyle adenine (BA) and 2, 4-dichlorophenoxy acetic acid (2, 4-D; 2.0 mg l− 1) were used for callus induction. The control light (16/8 h) produced optimum callogenic response (92.73%) than other colored lights. Compared to other colored lights, control grown cultures displayed maximum biomass accumulation (5.78 g l− 1) during a prolonged log phase at the 18th day of growth kinetics. Cultures grown under blue light enhanced total phenolic content (TPC; 102.32 μg/g DW), total flavonoid content (TFC; 22.07 μg/g DW) and total antioxidant capacity (TAC; 11.63 μg/g DW). On the contrary, green and red lights improved reducing power assay (RPA; 0.71 Fe(II) g− 1 DW) and DPPH-radical scavenging activity (DRSA; 80%). Herein, we concluded that the utilization of colored lights is a promising strategy for enhanced production of antioxidant secondary metabolites in callus cultures of S. rebaudiana.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 154, January 2016, Pages 51–56