In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application

• Andean blackberry leaf mediated biosynthesis of silver nanoparticles is reported.
• TEM–DLS analysis confirmed that the average size is 28.7 ± 12.9 nm.
• No cytotoxicity on the Hep-G2 cells by silver nanoparticles (0.01 μM to 1.0 μM)
• > 70% antioxidant efficacy was observed against DPPH.

In this article, biosynthesis of silver nanoparticles (AgNPs) using Andean Mora (Rubus glaucus Benth.) leaf has been reported. Different analytical techniques including UV–vis spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were used for the characterization of AgNPs. The initial appearance of color change with the intense surface plasmon resonance (SPR) bands around 440–455 in UV–visible spectra revealing the formation of AgNPs. The TEM image showed the AgNPs to be anisotropic, quasi-spherical in shape with sizes in the range of 12–50 nm. On the other hand, XRD studies revealed the formation of face-centered cubic structure for AgNPs. The surface modified AgNPs showed no cytotoxicity at the concentration ranging from 0.01 μM to 1.0 μM on the Hepatic cancer (Hep-G2) cell line and observed antioxidant efficacy > 70% at the concentration 0.05 mM/0.20 mL against 1, 1-diphenyl-2-picrylhydrazyl. From the results obtained it is suggested that AgNPs could be used effectively in future drug delivery systems and other biomedical concerns.

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