Synthesis of new chiral heterocyclic Schiff base modulated Cu(II)/Zn(II) complexes: Their comparative binding studies with CT-DNA, mononucleotides and cleavage activity

New Schiff base ligand L derived from the condensation reaction of 2-amino-3-formylchromone with (R)-2-amino-2-phenylethanol was synthesized and characterized which involves combination element of ammine functionality and naturally occurring heterocyclic chromone, 4H-benzopyran-4-one. Subsequently, their complexes 1 and 2 with Cu(NO3)2 and Zn(NO3)2, respectively were prepared. The DNA binding studies of the ligand L and complexes 1 and 2 with CT-DNA as compared to classical anticancer drug cisplatin were carried out by employing different optical methods viz, UV–vis, fluorescence, circular dichroism and viscosity measurements. Furthermore, the absorption studies, 1H and 31P with mononucleotides were also monitored to examine the base specific interactions of the transition metal complexes which revealed a higher propensity of copper(II) complex 1 for 5′-GMP while for zinc(II) complex 2 towards 5′-TMP involving groove binding mechanism of the complexes towards DNA. The complex 1 exhibits a remarkable DNA cleavage activity with pBR322 DNA in presence of different activators and cleavage reaction involves various oxygen species suggesting the involvement of active oxygen species for the DNA scission.

► Synthesis of Schiff base ligand and metal complexes.
► Interaction studies with CT-DNA.
► DNA binding affinity of Cu complex (1) is higher than cisplatin.