Phyto mediated biogenic synthesis of silver nanoparticles using leaf extract of Andrographis echioides and its bio-efficacy on anticancer and antibacterial activities

• Polyphenol constituents present in the leaf extract of A. echioides responsible for the capping of AgNPs with size ranges from 68.06 nm to 91.28 nm.
• Pentagonal and hexagonal silver nanostructures showed pronounced antiproliferative activity against MCF-7 cell lines.
• AgNPs showed efficient antibacterial activity against Escherichia coli and Staphylococcus aureus.

The present study reveals the efficiency of Andrographis echioides for green synthesis of silver nanoparticles (AgNPs). The leaf aqueous extract of A. echioides was used for the synthesis of AgNPs and they were characterized by UV–visible, High Resonance Scanning Electron Microscope (HRSEM), Energy-Dispersive X-ray Spectroscopy (EDX), Atomic Force Microscope (AFM), X-ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The toxicity of AgNPs was evaluated by using MTT assay. Our present study showed that biosynthesized AgNPs inhibited proliferation of human breast adenocarcinoma cancer cell line (MCF-7) with 31.5 μg/mL at 24 h incubation. Results suggest that AgNPs may exert its anticancer activity on MCF-7 cell line by suppressing its growth. The silver nanoparticles was studied against Gram positive and Gram negative bacteria. The highest antibacterial activity was found against Escherichia coli (28 mm) and Staphylococcus aureus (23 mm) respectively.

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