کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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30509 | 44483 | 2012 | 6 صفحه PDF | دانلود رایگان |
The development of an enzyme based biosensing method is described for evaluating the toxicity of solutions treated by titanium dioxide photocatalysis. The method is based on the potential of rat liver microsomal glutathione transferase ability (mGST) to get enhanced in the conditions of chemical and oxidative toxicity. Phenol is taken as a model pollutant due to its toxicity and prevalence in industrial processes. Chemical analysis of the parent compound, products and acute toxicity assays using the mGST activity, were conducted during and after the various photocatalytic treatments. The maximum mGST activity was observed from 60 and 120 min treated samples. This post-treatment toxicity might be due to toxic phenolic products, which may include p-benzoquinone, hydroquinone, benzenetriol and other intermediates. The enzymatic activity pattern observed after photocatalytic treatment corresponded well with the chemical degradation data obtained by HPLC-UV. The mGST assay seems to be an easy to use and promising approach for evaluating the effectiveness of wastewater treatment processes.
Figure optionsDownload as PowerPoint slideHighlights
► Enzymatic method for evaluating the toxicity of the effluent treated by nano-TiO2.
► Reactive intermediates influence the microsomal glutathione transferase activities.
► Confirmation of the evolution of reactive intermediates by HPLC/UV.
► Positive correlation between evolution of reactive species and modified activities.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 110, 2 May 2012, Pages 22–27