کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3070084 | 1580719 | 2009 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
CD44 is expressed in non-myelinating Schwann cells of the adult rat, and may play a role in neurodegeneration-induced glial plasticity at the neuromuscular junction
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کلمات کلیدی
CD44NMJERBBNeuromuscular junction - اتصال عصبی عضلانیamyotrophic lateral sclerosis - اسکلروز جانبی آمیوتروفیکFRET - انتقال انرژی رزونانسی فورسترALS - بیماری اسکلروز جانبی آمیوتروفیکReinnervation - تازه کردنstimulated emission depletion - تحریک تخلیه انتشارAxonal sprouting - جوانه زنیDenervation - حفاظتSchwann cell - سلول شوانSTED - محلConfocal microscopy - میکروسکوپ کانفوکال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
CD44 is a multifunctional cell surface glycoprotein which regulates cell-cell and cell-matrix interactions in a variety of tissues. In particular, the protein was found to be expressed in glial cells of developing, but not adult, peripheral nerves, where it takes part in signaling mediated by ErbB class of receptors for neuregulins. Here, we demonstrate, using high resolution morphological methods, tissue fractionation and RT-PCR, that CD44 is strongly expressed in terminal Schwann cell (TSC) at the neuromuscular junction (NMJ) of the adult rat skeletal muscle. As CD44 is also expressed by Schwann cells of the non-myelinated Remak bundles of the proximal peripheral nerves, it appears to be a marker of non-myelinating Schwann cell subpopulation. The analysis of transgenic rats bearing a mutated superoxide-dismutase gene (SOD1G93A) causing familial amyotrophic lateral sclerosis (ALS) revealed that TSC activation and morphological plasticity at the NMJ, caused by ongoing denervation-reinnervation is associated with a strong increase in CD44 expression therein. Notably, CD44 immunoreactivity is present in fine axon-escheating processes of the glial cells that guide reinnervation. In addition, we found that both in normal and SOD1G93A muscle, CD44 expressed in TSC partially colocalizes with immunoreactivities of neuregulin receptors ErbB2 and ErbB3. The colocalization appears to reflect a physical interaction, as evidenced by co-immunoprecipitation and fluorescence resonance energy transfer (FRET) analysis between CD44 and ErbB3. Importantly, TSC activation upon ALS-like neurodegeneration results in significant increase in molecular proximity of CD44 and ErbB3, which may have an impact on glial plasticity at the NMJ.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 34, Issue 2, May 2009, Pages 245-258
Journal: Neurobiology of Disease - Volume 34, Issue 2, May 2009, Pages 245-258
نویسندگان
Adam Gorlewicz, Jakub Wlodarczyk, Ewa Wilczek, Maciej Gawlak, Anna Cabaj, Henryk Majczynski, Klaudia Nestorowicz, Magdalena Aneta Herbik, Pawel Grieb, Urszula Slawinska, Leszek Kaczmarek, Grzegorz M. Wilczynski,