کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3070857 1580749 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Partial mitochondrial complex I inhibition induces oxidative damage and perturbs glutamate transport in primary retinal cultures.: Relevance to Leber Hereditary Optic Neuropathy (LHON)
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی عصب شناسی
پیش نمایش صفحه اول مقاله
Partial mitochondrial complex I inhibition induces oxidative damage and perturbs glutamate transport in primary retinal cultures.: Relevance to Leber Hereditary Optic Neuropathy (LHON)
چکیده انگلیسی

Leber Hereditary Optic Neuropathy (LHON) is a maternally inherited form of visual loss, due to selective degeneration of retinal ganglion cells. Despite the established aetiological association between LHON and mitochondrial DNA mutations affecting complex I of the electron transport chain, the pathophysiology of this disorder remains obscure. Primary rat retinal cultures were exposed to increasing concentrations of rotenone to titrate complex I inhibition. Neural cells were more sensitive than Müller glial cells to rotenone toxicity. Rotenone induced an increase in mitochondrial-derived free radicals and lipid peroxidation. Sodium-dependent glutamate uptake, which is mostly mediated by the glutamate transporter GLAST expressed by Müller glial cells, was reduced dose-dependently by rotenone with no changes in GLAST expression. Our findings suggest that complex I-derived free radicals and disruption of glutamate transport might represent key elements for explaining the selective retinal ganglion cell death in LHON.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 24, Issue 2, November 2006, Pages 308–317
نویسندگان
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