کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3120806 1583302 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
Postantifungal-like effect of sublethal treatment of Candida albicans with acid-electrolyzed water
چکیده انگلیسی


• Dilute acid electrolyzed water (AEW) exerted postantifungal like effect.
• The fungal growth delay caused by dilute AEW is due to reactive oxygen species.
• The hydroxyl radical is formed from intracellular hypochlorous acid.
• Partially inactivated AEW could be used for the treatment of oral candidiasis.

ObjectiveAcid-electrolyzed water (AEW) has been applied to the treatment of oral candidiasis. We evaluated the postantifungal effect (PAFE)-like activity of AEW against Candida albicans under sublethal conditions by exposing C. albicans to dilute AEW.DesignThe growth of C. albicans after a short-term exposure to dilute AEW was evaluated in broth and on agar culture. The involvement of reactive oxygen species (ROS) in the PAFE was examined by flow cytometric analysis with hydroxyphenyl fluorescein (HPF) as a fluorescence probe.ResultsThe dilute AEW exerted PAFE-like activity against C. albicans. ROS were produced in the cells treated with AEW diluted 16 times or fewer. The increase in HPF fluorescence after treatment with dilute AEW was cancelled by dimethyl sulfoxide, a hydroxyl radical (OH) scavenger.ConclusionIt would be expected that the ROS, especially OH, produced in the C. albicans cells treated with sublethal dilutions of AEW could exert PAFE-like activity against the fungal cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 60, Issue 3, March 2015, Pages 479–487
نویسندگان
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