کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3121176 1583366 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effects of nicotine and cotinine on Porphyromonas gingivalis colonisation of epithelial cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
The effects of nicotine and cotinine on Porphyromonas gingivalis colonisation of epithelial cells
چکیده انگلیسی

Smoking is a risk factor for development of periodontitis. Porphyromonas gingivalis is an important colonizer of the subgingival crevice and is a major pathogenic agent in the initiation and progression of severe forms of periodontal disease. However, the effect of major cigarette's derivatives on P. gingivalis is poorly understood. The purpose of this study was to determine the influence of nicotine and cotinine on bacterial colonisation to epithelial cells. KB cells monolayers and P. gingivalis ATCC 33277 were exposed to 0.1, 10 and 100 μg/mL of nicotine and cotinine concentrations. The epithelial cells were incubated for 24 h while P. gingivalis was exposed to these substances until reach early logarithmic phase. After the incubation period, P. gingivalis ability to colonize KB cells was assayed. The number of cell-associated/invasive bacteria was assessed by counting the colony-forming unities. 100 μg/mL cotinine significantly increased P. gingivalis association and invasion of epithelial cells, when the bacteria was exposed to this substance (p < 0.05; ANOVA – Tukey test). No other condition or drug altered the bacteria colonisation ability (p > 0.05). These data indicated that cotinine may interfere with P. gingivalis ability to associate and invade the epithelial cells. Further studies are needed to investigate whether oral cells might be more susceptible to be colonized by P. gingivalis in smokers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 54, Issue 11, November 2009, Pages 1061–1067
نویسندگان
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