کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3121942 1583413 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RNA integrity and in situ RT-PCR in dento-alveolar tissues after microwave accelerated demineralisation
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
پیش نمایش صفحه اول مقاله
RNA integrity and in situ RT-PCR in dento-alveolar tissues after microwave accelerated demineralisation
چکیده انگلیسی

SummaryObjectiveThe structural organization of oral soft tissue and its relationship with highly calcified teeth are difficult to preserve unless tissues are decalcified, paraffin embedded and subsequently sectioned. However, enamel decalcification time and its negative impact on RNA integrity makes it difficult to effectively analyse in situ gene expression. This study examined the impact of microwave-enhanced decalcification on processing time, RNA integrity and detection of in situ mRNA expression in hard and soft tissue for cell type specific markers of Keratinocyte growth factor receptor, Scleraxis and Osteonectin.DesignMaxillas and mandibles were obtained from three male Wistar strain rats. Right side tissues were decalcified using a microwave plus 10% EDTA solution (M+) while left side tissues were decalcified in 10% EDTA solution alone (M−).ResultsMicrowave use reduced decalcification time by up to 50% and had no significant impact on morphology, RNA quality and in situ detection of gene expression relative to the M-group.ConclusionsIn situ RT-PCR gene expression of microwave decalcified paraffin-embedded oral tissues is an effective technique to localize in situ gene expression while maintaining excellent soft and hard tissue architecture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Oral Biology - Volume 51, Issue 2, February 2006, Pages 164–169
نویسندگان
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