کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3142515 1196787 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High doses of bisphosphonates reduce osteoblast-like cell proliferation by arresting the cell cycle and inducing apoptosis
ترجمه فارسی عنوان
دوزهای بالایی از بیسفسفوناتها باعث کاهش تکثیر سلولهای استئوبلاست میشوند با توقف چرخه سلولی و ایجاد آپوپتوز
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی دندانپزشکی، جراحی دهان و پزشکی
چکیده انگلیسی

ObjectivesThe study objective was to evaluate the effect on osteoblast growth of high concentrations of three nitrogen-containing bisphosphonates (pamidronate, alendronate, and ibandronate) and one non-nitrogen-containing bisphosphonate (clodronate), using the MG-63 cell line as an osteoblast model, in order to determine the role of osteoblasts in bisphosphonate-related osteonecrosis of the jaw (BRONJ).Materials and methodsOsteoblasts were incubated in culture medium with different doses of pamidronate, alendronate, ibandronate or clodronate. The proliferative capacity of the osteoblasts was determined by spectrophotometry (MTT-based) at 24 h of culture. Flow cytometry was used to determine the percentage of cells in each cell cycle phase (G0/G1, G2/M, and S) and to discriminate apoptotic cell death from necrotic cell death in the cell cycle at 24 h of treatment.ResultsAll the bisphosphonates assayed produced a significant and dose-dependent reduction in MG-63 proliferation at the high doses assayed (10−4 and 5 × 10−5 M) in comparison with controls (p <0.001). Cell cycle study revealed that all assayed bisphosphonates significantly arrested the cell cycle in phase G0/G1 at doses of 10−4 and 5 × 10−5 M, increasing the percentage of cells in this phase (p <0.05). Apoptosis/necrosis studies showed significant changes compared with control cells, with an increased percentage of cells in apoptosis after treatment with 10−4 or 5 × 10−5 M of pamidronate, alendronate, ibandronate, or clodronate (p <0.05).ConclusionsHigh doses of nitrogen-containing or non-nitrogen-containing bisphosphonates can reduce the proliferation of MG-63 osteoblast-like cells by arresting the cell cycle and inducing apoptosis/necrosis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Cranio-Maxillofacial Surgery - Volume 43, Issue 3, April 2015, Pages 396–401
نویسندگان
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