کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3257151 | 1207394 | 2012 | 11 صفحه PDF | دانلود رایگان |
Immunosuppression resulting from excessive post-trauma apoptosis of hyperactivated T cells is controversial. TRAIL mediated T cell apoptosis decreases highly activated T cells' responses. Caspase-10, a particular TRAIL target, was increased in trauma patients' T cells with concomitantly elevated plasma TRAIL levels. These patients' T cells developed anergy, implicating increased TRAIL-mediated T cell apoptosis in post-trauma T cell anergy. Control T cells cultured with patients' sera containing high TRAIL levels increased their caspase-10 activity and apoptosis. Stimulated primary T cells are TRAIL apoptosis resistant. Increased plasma thrombospondin-1 and T cell expression of CD47, a thrombospondin-1 receptor, preceded patients' T cell anergy. CD47 triggering of T cells increased their sensitivity to TRAIL-induced apoptosis. Augmentation of T cell TRAIL-induced apoptosis was secondary to CD47 triggered activation of the Src homology-containing phosphatase-1 (SHP-1) and was partially blocked by a SHP-1 inhibitor. We suggest that combined post-trauma CD47 triggering, SHP-1 mediated NFκB suppression, and elevated TRAIL levels increase patients' CD47 expressing T cell apoptosis, thus contributing to subsequent T cell anergy.
► Trauma patients' T cells with elevated apoptosis and CD47 expression become anergic.
► Only patients with high plasma TRAIL and TSP-1 developed T cell apoptosis then anergy.
► TRAIL and TSP-1 containing patient plasma induced TRAIL apoptosis in control T cells.
► Control T cells were sensitive to TRAIL mediated apoptosis after CD47 triggering.
► CD47 triggered SHP-1's depression of NFκB to increase T cells' TRAIL apoptosis.
Journal: Clinical Immunology - Volume 145, Issue 1, October 2012, Pages 44–54