کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3311408 | 1210861 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hepatocyte nuclear factor-4α and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix
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کلمات کلیدی
ECMEHSsiRNAsdsRNAEngelbreth-Holm-SwarmHnf - HNFRNA interference - RNA تداخل کنندهdouble-stranded RNA - RNA دو رشته ایRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهsmall interfering RNAs - RNA های تداخل کوچکdigoxigenin - دیگوکسین ژنDIG - شماTranscription factor - عامل رونویسیhepatocyte nuclear factor - عامل هسته ای هپاتوسیتExtracellular matrix - ماتریکس خارج سلولیCollagen - کلاژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Hepatocyte nuclear factor-4α and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix Hepatocyte nuclear factor-4α and -1 small interfering RNA inhibits hepatocyte differentiation induced by extracellular matrix](/preview/png/3311408.png)
چکیده انگلیسی
Liver-specific genes and hepatocyte nuclear factor (HNF)-4α and -1 are coordinately regulated by extracellular matrix (ECM). However, still are unclear interactions between liver-specific genes and these liver-enriched transcription factors in the mechanism of hepatocyte differentiation regulated by ECM. To elucidate the relationship, we used small interfering RNA (siRNA), which obtains strong and specific knockdown of gene expression in cell culture. Treatment with siHNF-4α and siHNF-1 declined the expression levels for HNF-4α mRNA and HNF-1 mRNA in primary rat hepatocytes, respectively. The mRNA expressions of albumin, transthyretin, and apolipoproteins that were up-regulated in hepatocytes cultured on a basement membrane matrix, Engelbreth-Holm-Swarm (EHS) gel, were decreased in the presence of siHNF-4α or siHNF-1. Moreover, siHNF-4α and siHNF-1 did not affect the morphology and actin assembly of hepatocytes. These findings demonstrated that HNF-4α and HNF-1 directly regulate liver-specific gene expression and might be downstream of cytoskeletal organization in the mechanism by which the differentiated phenotype of hepatocytes is regulated by EHS gel.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hepatology Research - Volume 35, Issue 1, May 2006, Pages 3-9
Journal: Hepatology Research - Volume 35, Issue 1, May 2006, Pages 3-9
نویسندگان
Takayuki Kimata, Masahito Nagaki, Yoshihiko Tsukada, Tomio Ogiso, Hisataka Moriwaki,