کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3355985 1217228 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Burn injury induces the expression of cystine/glutamate transporter (xc−) in mouse T cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Burn injury induces the expression of cystine/glutamate transporter (xc−) in mouse T cells
چکیده انگلیسی

System xc− transporter, formed by the association of CD98 and xCT proteins, regulates the import of cystine into cells and is poorly expressed in T lymphocytes. Thermal injury is associated with high oxidative stress, decreased levels of glutathione (GSH) and protein deficiency, all described as promoters of xCT expression and system xc− activity. T cell dysfunction is a consequence of thermal injury and has been related to oxidative stress. In order to evaluate if thermal injury induced system xc− expression in splenic T lymphocytes, cells were isolated from sham- and burn-injured mice at day 10 post-burn and cultured in 2-mercaptoethanol (2-ME)-rich and -free media. Isolated splenic T cells were stimulated and cell proliferation, system xc− expression and cystine transport activity were measured. Our results demonstrate that only burn-injured T cells express xCT and proliferate in (2-ME)-free media. In these cells, viability and CD25 expression was higher than control T cells. xc− system expression was responsible for significantly higher 14C-cystine uptake by burn-injured T cells and its inhibition by sulfasalazine (SASP) decreased significantly their proliferation. Overall, these results demonstrate that xCT expression is induced by thermal injury in T lymphocytes and that cystine import by xc− leads to T cell dysfunction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 125, Issue 2, 15 August 2009, Pages 137–144
نویسندگان
, , , ,