کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3355993 | 1217229 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Possible contribution of DNase γ to immunoglobulin V gene diversification
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
Somatic hypermutation (SHM) diversifies the rearranged immunoglobulin variable (V) region gene in B cells, contributing to affinity maturation of antibodies. It is believed that SHM is generated either by direct replication or by error-prone repair systems resolving V region DNA lesions caused directly or indirectly by cytidine deaminase AID. In accord with a part of these mechanisms, it was reported that SHM is associated with staggered double-strand DNA breaks (DSBs) occurring in the rearranged V regions. However, endonucleases responsible for the DSBs remain elusive. Here we show that DNase γ, a member of DNase I family endonucleases, contributes to the generation of SHM including point mutation, and nucleotide insertion and deletion in chicken DT40 B cell line. DNase γ also contributes to the generation of staggered DSBs in the rearranged V region. These results raise a possibility that DNase γ is involved in the V gene mutation machinery.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunology Letters - Volume 125, Issue 1, 30 June 2009, Pages 22-30
Journal: Immunology Letters - Volume 125, Issue 1, 30 June 2009, Pages 22-30
نویسندگان
Noriaki Okamoto, Mariko Okamoto, Shinsuke Araki, Hiroshi Arakawa, Ryushin Mizuta, Daisuke Kitamura,