کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3415115 1224940 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Characterization of a 21 kDa protein from Trypanosoma cruzi associated with mammalian cell invasion
چکیده انگلیسی

Trypanosoma cruzi genomic database was screened for hypothetical proteins that showed high probability of being secreted or membrane anchored and thus, likely involved in host-cell invasion. A sequence that codes for a 21 kDa protein that showed high probability of being secreted was selected. After cloning this protein sequence, the results showed that it was a ubiquitous protein and secreted by extracellular amastigotes. The recombinant form (P21-His6) adhered to HeLa cells in a dose-dependent manner. Pretreatment of host cells with P21-His6 inhibited cell invasion by extracellular amastigotes from G and CL strains. On the other hand, when the protein was added to host cells at the same time as amastigotes, an increase in cell invasion was observed. Host-cell pretreatment with P21-His6 augmented invasion by metacyclic trypomastigotes. Moreover, polyclonal antibody anti-P21 inhibited invasion only by extracellular amastigotes and metacyclic trypomastigotes from G strain. These results suggested that P21 might be involved in T. cruzi cell invasion. We hypothesize that P21 could be secreted in the juxtaposition parasite-host cell and triggers signaling events yet unknown that lead to parasite internalization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 11, Issue 5, April 2009, Pages 563–570
نویسندگان
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