کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3415914 | 1224984 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel phase-variable autotransporter serine protease, AusI, of Neisseria meningitidis
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کلمات کلیدی
PBSORFEDDHAOD600apparent molecular massBSA - BSAbovine serum albumin - آلبومین سرم گاوAutotransporter - اتوبوسرانیoptical density at 600 nm - تراکم نوری در 600 نانومترPhase variation - تنوع فازOuter membrane - غشای خارجیopen reading frame - قاب خواندن بازPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNeisseria meningitidis - نایسریا مننژیتیس، مننگوکوکSecreted proteins - پروتئین های جدا شده
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
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چکیده انگلیسی
The sequenced genomes of pathogenic Neisseria meningitidis strains contain up to eight genes putatively encoding autotransporters, which are secreted proteins implicated in virulence. Here, we have characterized one of these genes, designated ausI, which encodes an autotransporter of the serine protease family. It was found to be specific for N. meningitidis and present in 14 out of 20 isolates, although only six of them expressed the gene. We show that expression of the gene is subject to phase variation as a result of a variable number of cytosines in a poly-C tract in the coding region. The open reading frame went out-of-phase at the poly-C tract in seven strains that did not express AusI. In the eighth strain, the open reading frame remained in frame at the poly-C tract, but it was disrupted by a premature stop codon further downstream. In accordance with its assignment as an autotransporter, a secreted AusI passenger domain was released into the extracellular milieu. This release was influenced by another autotransporter, NalP, as different forms of AusI were produced in the presence or absence of NalP. In silico sequence analysis suggested several putative functions for AusI, which, however, could not be confirmed experimentally.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbes and Infection - Volume 8, Issue 8, July 2006, Pages 2088-2097
Journal: Microbes and Infection - Volume 8, Issue 8, July 2006, Pages 2088-2097
نویسندگان
Peter van Ulsen, Ben Adler, Peter Fassler, Maarten Gilbert, Muriel van Schilfgaarde, Peter van der Ley, Loek van Alphen, Jan Tommassen,