کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3424026 | 1594226 | 2016 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Metabolic profiling during HIV-1 and HIV-2 infection of primary human monocyte-derived macrophages Metabolic profiling during HIV-1 and HIV-2 infection of primary human monocyte-derived macrophages](/preview/png/3424026.png)
Examine metabolic alterations made by HIV-1 and HIV-2 infections in macrophages.We also investigated the nucleotide metabolic impact made by Vpx in macrophages.HIVs generated differential metabolic profiles for nucleotides and quinolinate.
We evaluated cellular metabolism profiles of HIV-1 and HIV-2 infected primary human monocyte-derived macrophages (MDMs). First, HIV-2 GL-AN displays faster production kinetics and greater amounts of virus as compared to HIV-1s: YU-2, 89.6 and JR-CSF. Second, quantitative LC–MS/MS metabolomics analysis demonstrates very similar metabolic profiles in glycolysis and TCA cycle metabolic intermediates between HIV-1 and HIV-2 infected macrophages, with a few notable exceptions. The most striking metabolic change in MDMs infected with HIV-2 relative to HIV-1-infected MDMs was the increased levels of quinolinate, a metabolite in the tryptophan catabolism pathway that has been linked to HIV/AIDS pathogenesis. Third, both HIV-1 and HIV-2 infected MDMs showed elevated levels of ribose-5-phosphate, a key metabolic component in nucleotide biosynthesis. Finally, HIV-2 infected MDMs display increased dNTP concentrations as predicted by Vpx-mediated SAMHD1 degradation. Collectively, these data show differential metabolic changes during HIV-1 and HIV-2 infection of macrophages.
Journal: Virology - Volume 491, April 2016, Pages 106–114