کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3425777 | 1227299 | 2010 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Modulation of apoptosis and immune signaling pathways by the Hantaan virus nucleocapsid protein Modulation of apoptosis and immune signaling pathways by the Hantaan virus nucleocapsid protein](/preview/png/3425777.png)
Herein, we show a direct relationship between the Hantaan virus (HTNV) nucleocapsid (N) protein and the modulation of apoptosis. We observed an increase in caspase-7 and -8, but not -9 in cells expressing HTNV N protein mutants lacking amino acids 270–330. Similar results were observed for the New World hantavirus, Andes virus. Nuclear factor kappa B (NF-κB) was sequestered in the cytoplasm after tumor necrosis factor receptor (TNFR) stimulation in cells expressing HTNV N protein. Further, TNFR stimulated cells expressing HTNV N protein inhibited caspase activation. In contrast, cells expressing N protein truncations lacking the region from amino acids 270–330 were unable to inhibit nuclear import of NF-κB and the mutants also triggered caspase activity. These results suggest that the HTNV circumvents host antiviral signaling and apoptotic response mediated by the TNFR pathway through host interactions with the N protein.
Journal: Virology - Volume 401, Issue 2, 5 June 2010, Pages 165–178