کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3426696 1227341 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ویروس شناسی
پیش نمایش صفحه اول مقاله
Functional characterization of ex vivo blood myeloid and plasmacytoid dendritic cells after infection with dengue virus
چکیده انگلیسی

Myeloid and plasmacytoid dendritic cells (mDC and pDC) are naturally distinctive subsets. We exposed both subsets to dengue virus (DV) in vitro and investigated their functional characteristics. High levels of DV replication in mDC were found to correlate with DC-SIGN expression. Production of inflammatory cytokines by mDC increased gradually after DV-infection, which was dependent on DV replication. Co-stimulatory markers were upregulated on mDC upon DV-infection. On the contrary, lower levels of DV-replication were observed in pDC, but the cytokine production in pDC was quicker and stronger. This cytokine response was not dependent on viral replication, but dependent on cell endosomal activity and TLR7, and could be also induced by purified DV genome RNA. These results clearly suggested functional differences between mDC and pDC in response to DV infection. Additionally, the TLR7-mediated recognition of DV RNA may be involved in pDC functional activation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 383, Issue 2, 20 January 2009, Pages 207–215
نویسندگان
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