کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3841252 1247968 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Infrared spectroscopy: a reagent-free method to distinguish Alzheimer's disease patients from normal-aging subjects
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی پزشکی و دندانپزشکی (عمومی)
پیش نمایش صفحه اول مقاله
Infrared spectroscopy: a reagent-free method to distinguish Alzheimer's disease patients from normal-aging subjects
چکیده انگلیسی

The physiopathogenesis of Alzheimer's disease (AD) is related to various biochemical mechanisms that may be reflected by changes in plasma components. In the current study, Fourier transform-infrared (FT-IR) spectroscopy was used to identify these biochemical variations by monitoring spectral differences in the plasma of 40 AD patients compared with those of 112 control subjects. A hierarchical classification in the whole mid-infrared region allowed a clear separation between AD and controls (C) that was optimized by using a restricted spectral range (1480–1428 cm−1). Spectral changes confirmed vibration differences between AD and C mostly related to modified lipid and nucleic acid structures involved in oxidative stress-dependent processes of AD. Moreover, the analysis of samples in the 1480–910-cm−1 region allowed the distinction between C and AD with an accuracy of 98.4% and showed 2 subgroups C1 and C2 within the C group. Interestingly, the C1 subgroup was located closer to the AD group than the C2 subgroup, which suggests biochemical differences within the nondemented subjects. Biochemical studies revealed a significant increase in a specific marker of oxidative stress, F8-isoprostanes (8-epi-PGF2α) levels, in the plasma of AD patients as compared with total controls and subgroup C2 but not subgroup C1. Thus, these results suggest that use of FT-IR spectroscopy could be valuable to distinguish AD patients from normal-aging subjects.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Translational Research - Volume 152, Issue 3, September 2008, Pages 103–112
نویسندگان
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