کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4327353 | 1614120 | 2010 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitization of rat facial cutaneous mechanoreceptors by activation of peripheral N-methyl-d-aspartate receptors
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کلمات کلیدی
N-methyl-d-aspartate receptor subunit 2BAMPACGRPEC50NMDAN-methyl-d-aspartateAPVNGSCy3FITCPGP 9.5PBSiGluRsNR2B2-amino-5-phosphonovalerate - 2-آمینو-5-فسفونوالراتMechanical threshold - آستانه مکانیکیα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid - اسید α-آمینو 3-هیدروکسی-5-متیل-4-ایزوکسول پپونیکanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاConduction velocity - سرعت هدایتnormal goat serum - سرم طبیعی بزCyanine - سیانینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتSubstance P - ماده PPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریRat - موش صحراییhalf maximal effective concentration - نیمه حداکثر غلظت موثرpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازprotein gene product - پروتئین محصول ژنcalcitonin gene-related peptide - پپتید مرتبط با ژن کلسی تونینKainate - کیناتglutamate - گلوتاماتionotropic glutamate receptors - گیرنده های گلوتامات یوناتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
The effect of subcutaneous injection of glutamate on the mechanical sensitivity of rat facial cutaneous mechanoreceptors was examined. Individual facial mechanoreceptors were recorded in the trigeminal ganglion of anesthetized Sprague-Dawley rats. An electronic von Frey hair was used to measure the mechanical threshold (MT) of the afferent fibers at baseline and following subcutaneous injection of glutamate (0, 0.01, 0.1, 1 M; 10 μl) or glutamate (0, 0.1 M) plus the competitive N-methyl-d-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonovalerate (APV; 0.01 M). Subcutaneous injections were randomized and the investigator was unaware of their content. Changes in MT were assessed with a repeated measure ANOVA with time, sex and treatment as factors. Immunohistochemistry was used to confirm NMDA receptor expression by cutaneous nerve fibers. A total of 100 (50 per sex) facial mechanoreceptors were recorded from 61 (32 females, 29 males) rats in two separate experiments. Subcutaneous injections of higher concentrations of glutamate (1, 0.1 M) induced a significant mechanical sensitization of skin afferent fibers (compared to 0 and 0.01 M). Females (EC50 = 16.2 mM) were more sensitive to glutamate than males (EC50 = 73.0 mM). Facial cutaneous nerve fibers in both sexes expressed NMDA receptors. APV blocked the mechanical sensitization of the afferent fibers treated by glutamate 0.1 M in both sexes with a lower effect in females at a 10-20 minute post-injection. Subcutaneous injection of glutamate mechanically sensitizes rat facial cutaneous mechanoreceptors through activation of peripheral NMDA receptors. Peripheral NMDA receptor antagonists may be considered for craniofacial pain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1319, 10 March 2010, Pages 70-82
Journal: Brain Research - Volume 1319, 10 March 2010, Pages 70-82
نویسندگان
Parisa Gazerani, Xudong Dong, Mianwei Wang, Ujendra Kumar, Brian E. Cairns,