کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4327596 | 1614137 | 2009 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purpurin is a key molecule for cell differentiation during the early development of zebrafish retina
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کلمات کلیدی
GAP-43DPFGFAPONLINLFGFHDAChpfPKCHSPGbromo-deoxyuridine - برومو دزوویریدینBrdU - بروموداکسی اوریدینRetinoic acid - رتینوئیک اسیدDevelopment - رشدgrowth associated protein-43 - رشد پروتئینی مرتبط با آن 43days post-fertilization - روز پس از لقاحhours post-fertilization - ساعت پس از لقاحRetina - شبکیهfibroblast growth factor - فاکتور رشد فیبروبلاستouter nuclear layer - لایه بیرونی هسته ایinner nuclear layer - لایه داخلی هسته ایmorpholino - مورفولینوNeurogenesis - نوروژنزHeparan sulfate proteoglycan - هپارین سولفات پروتئگلیکانhistone deacetylase - هیستون داستیلازGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالProtein kinase C - پروتئین کیناز سیPurpurin - پورپورینZebrafish - گورخرماهی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Recently, we cloned purpurin cDNA as an upregulated gene in the axotomized fish retina. The retina-specific protein was secreted from photoreceptors to ganglion cell layer during an early stage of optic nerve regeneration in zebrafish retina. The purpurin worked as a trigger molecule for axonal regrowth in adult injured fish retina. During zebrafish development, purpurin mRNA first appeared in ventral retina at 2Â days post-fertilization (dpf) and spread out to the outer nuclear layer at 3Â dpf. Here, we investigated the role of purpurin for zebrafish retinal development using morpholino gene knockdown technique. Injection of purpurin morpholino into the 1-2 cell stage of embryos significantly inhibited the transcriptional and translational expression of purpurin at 3Â dpf. In the purpurin morphant, the eyeball was significantly smaller and retinal lamination of nuclear and plexiform layers was not formed at 3Â dpf. Retinal cells of purpurin morphants were still proliferative and undifferentiated at 3Â dpf. The visual function of purpurin morphant estimated by optomotor response was also suppressed at 5Â dpf. By contrast, the control morphants with random sequence morpholino showed retinal lamination with distinct layers and differentiated cells at 3Â dpf. These results strongly suggest that purpurin is a key molecule for not only optic nerve regeneration in adult but also cell differentiation during early development in embryo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1302, 20 November 2009, Pages 54-63
Journal: Brain Research - Volume 1302, 20 November 2009, Pages 54-63
نویسندگان
Mikiko Nagashima, Kazuhiro Mawatari, Masayuki Tanaka, Tomomi Higashi, Hikaru Saito, Ken-ichiro Muramoto, Toru Matsukawa, Yoshiki Koriyama, Kayo Sugitani, Satoru Kato,