کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4355886 | 1615646 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of cell death pathways in the inner ear of the aging CBA/J mouse
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کلمات کلیدی
PBSEndonuclease GJnkSAPKc-Jun-N-terminal kinaseABRMAPK - MAPKROS - ROSEndo G - آندو گAge-related hearing loss - افت شنوایی مرتبط با سنOxidative stress - تنش اکسیداتیوCochlea - حلزون گوشApoptosis - خزان یاختهایPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریStress-activated protein kinase - پروتئین کیناز فعال شده با استرسPropidium iodide - پروتئین یدیدCalpain - کالپینcaspase - کسپاز یا کاسپازmitogen-activated protein kinases - کیناز پروتئین فعال MitogenReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
سیستم های حسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have previously demonstrated that oxidative stress increases in the inner ear of aging CBA/J mice and might contribute to the loss of function of the sensory system. We now investigate the activation of cell death pathways in the cochleae of these animals. Middle-aged (12Â months) and old (18-26Â months) mice with hearing deficits displayed outer hair cell nuclei with apoptotic and, to a lesser extent, necrotic features. Both intrinsic and extrinsic cell death pathways were activated by translocation or post-translational modification of proteins in the aging cochlea as compared to young (3Â months) animals. Cytosolic cytochrome c increased, formed a complex with, and activated caspase 9. Endonuclease G translocated to the nuclei of aging outer hair cells suggesting its function as an apoptotic DNase. The cleaved (and hence active) forms of calpain I and calpain II increased while active cathepsin D was transiently elevated in middle-aged but not old animals. Finally, increases in the phosphorylation of p38 MAPK and JNK implicated the additional involvement of the MAPK pathway. The results suggest that multiple cell death pathways, all potentially linked to oxidative stress, are activated in hair cells of the auditory organ in aging mice.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Hearing Research - Volume 254, Issues 1â2, 11 August 2009, Pages 92-99
Journal: Hearing Research - Volume 254, Issues 1â2, 11 August 2009, Pages 92-99
نویسندگان
Su-Hua Sha, Fu-Quan Chen, Jochen Schacht,