کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4411066 1307577 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Toxic responses in primary rat hepatocytes exposed with occupational dust collected from work environment of bone-based industrial unit
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Toxic responses in primary rat hepatocytes exposed with occupational dust collected from work environment of bone-based industrial unit
چکیده انگلیسی

In this in vitro study we investigated the toxic responses in hepatocytes treated with occupational dust to which workers are exposed in bone-based industrial units. The present study investigated the toxicity mechanism of bone-based occupational dust, from a particular industrial unit, on isolated rat hepatocytes. The hepatocytes were isolated by collagenase perfusion method and cell viability was determined by trypan blue exclusion and MTT [3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay treated with occupational dust at 0.1–1.0 mg mL−1, for 120 min. The cell viability decreased significantly in a concentration-dependent manner. Dust induced significant membrane damage measured by lactate dehydrogenase (LDH) and glutathione (GSH) release in culture media for 30-, 60- and 120 min treatment duration. The toxicity was found to be correlated with the induction of lipid peroxidation (LPO). In addition, nitric oxide (NO), and hydrogen peroxide (H2O2) generation by occupational dusts were also found to be time- and concentration-dependent. Over all the present study provides initial evidences for the toxic potential of occupational dust generated in bone-based industries and, therefore, the dust exposure to workers in unorganized industrial units should be controlled.

Research highlights
► Dust collected from the work environment of 12 bone-based industrial units.
► A preliminary haemolytic potential of dust particles from these units carried out.
► Toxicity assessment of dust particles conducted on rat hepatocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 83, Issue 4, April 2011, Pages 455–460
نویسندگان
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