کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4481664 | 1623116 | 2014 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Toxicity assessment of chlorinated wastewater effluents by using transcriptome-based bioassays and Fourier transform mass spectrometry (FT-MS) analysis Toxicity assessment of chlorinated wastewater effluents by using transcriptome-based bioassays and Fourier transform mass spectrometry (FT-MS) analysis](/preview/png/4481664.png)
• Whole chlorinated effluent toxicity was assessed by bioassays and FT-MS analysis.
• Chlorination elevated the cytotoxicity of activated sludge effluent.
• Chlorination reduced the cytotoxicity of membrane bioreactor effluent.
• Toxicity was dependent on effluent organic matter and disinfection by-products.
Effects of chlorination on the toxicity of wastewater effluents treated by activated sludge (AS) and submerged membrane bioreactor (S-MBRB) systems to HepG2 human hepatoblastoma cells were investigated. In addition to the cytotoxicity and genotoxicity assays, the DNA microarray-based transcriptome analysis was performed to evaluate the change in types of biological impacts on HepG2 cells of the effluents by chlorination. Effluent organic matter (EfOM) and disinfection by-products (DBPs) were also characterized by using Fourier transform mass spectrometry (FT-MS). Although no significant induction of genotoxicity was observed by chlorination for both effluents, the chlorination elevated the cytotoxicity of AS effluent but reduced that of S-MBRB effluent. The FT-MS analyses revealed that more DBPs including nitrogenated DBPs (N-DBPs) were formed in the AS effluent than in the S-MBRB effluent by chlorination, supporting the increased cytotoxicity of AS effluent. The lower O/C ratio of S-MBRB EfOM suggests that a large number of organic molecules were detoxified by chlorination, which consequently decreased the cytotoxicity of S-MBRB effluent. Integration of all the results highlights that both cytotoxicity and biological impacts of chlorinated wastewater effluents were clearly dependent on the EfOM characteristics such as DBPs and O/C ratio, namely, on types of treatment systems.
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Journal: Water Research - Volume 52, 1 April 2014, Pages 73–82