Fractional, biodegradable and spectral characteristics of extracted and fractionated sludge extracellular polymeric substances

Correlation between fractional, biodegradable and spectral characteristics of sludge extracellular polymeric substances (EPS) by different protocols has not been well established. This work extracted sludge EPS using alkaline extractants (NH4OH and formaldehyde + NaOH) and physical protocols (ultrasonication, heating at 80 °C or cation exchange resin (CER)) and then fractionated the extracts using XAD-8/XAD-4 resins. The alkaline extractants yielded more sludge EPS than the physical protocols. However, the physical protocols extracted principally the hydrophilic components which were readily biodegradable by microorganisms. The alkaline extractants dissolved additional humic-like substances from sludge solids which were refractory in nature. Different extraction protocols preferably extracted EPS with distinct fractional, biodegradable and spectral characteristics which could be applied in specific usages.

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► Fractionation of the sludge EPS extracted by physical and chemical protocols.
► Alkaline extractions yielded more crude EPS than did physical protocols.
► The aromatic, carboxylic and hydrocarbon were preferentially extracted by alkaline.
► Aliphatic materials could be efficiently extracted by physical protocols.
► EPS by physical extraction showed higher BDOC/DOC ratios than that by alkaline.