Bio-reaction of nitrobenzene with Microcystis aeruginosa: Characteristics, kinetics and application

The bio-reaction of nitrobenzene (NB) with Microcystis aeruginosa was investigated at different initial algal densities and NB concentrations by performing static experiments. The results showed that the elimination of NB was enhanced by the bio-reaction, and the reaction rate varied as a function of the reaction time. Moreover, the reaction rate was significantly affected by the algal density and NB concentration. A kinetic analysis showed that the elimination of NB in a solution without algae appeared to be pseudo-first-order with respect to the NB concentration, whereas a first-order model was too oversimplified to describe the elimination of NB in a solution with algae. Assuming that different algal cells have the same effect on the bio-reaction under the same conditions, the bio-reaction can be described as dCNB = −k0CAmANBndt (where k0 is the reaction rate constant, CA is the algae density and CNB is the concentration of NB). When the growth of algae was not considered, the value of k0, m and n were 8.170 × 10−4, 0.5887 and 1.692, respectively. Alternatively, when algae were in the exponential growth phase, the value of k0, m and n were 1.6871 × 10−5, 0.7248 and 2.5407, respectively, according to a nonlinear fitting analysis. The kinetic model was also used to elucidate the effect of nutritional limitation on the bio-reaction.

How do environmental factors affect the bio-reaction of nitrobenzene with Microcystis aeruginosa?Figure optionsDownload high-quality image (185 K)Download as PowerPoint slideHighlights
► High concentration of NB and M. aeruginosa favor the bio-reaction.
► The elimination of NB in the solution without algae follows a first-order reaction.
► Develop an empirical kinetic model to fit the elimination of NB in algal solution.
► Nutrition limitation does not affect bio-reaction directly analyzed by kinetic model.