کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4485579 1316957 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فرآیندهای سطح زمین
پیش نمایش صفحه اول مقاله
The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth
چکیده انگلیسی

Emerging nanomaterials are of great concern to wastewater treatment utilities and the environment. The inhibitory effects of silver nanoparticles (Ag NPs) and other important Ag species on microbial growth were evaluated using extant respirometry and an automatic microtiter fluorescence assay. Using autotrophic nitrifying organisms from a well-controlled continuously operated bioreactor, Ag NPs (average size=14±6 nm), Ag+ ions (AgNO3), and AgCl colloids (average size=0.25 μm), all at 1 mg/L Ag, inhibited respiration by 86±3%, 42±7%, and 46±4%, respectively. Based on a prolonged microtiter assay, at about 0.5 mg/L Ag, the inhibitions on the growth of Escherichia coli PHL628-gfp by Ag NPs, Ag+ ions, and AgCl colloids were 55±8%, 100%, and 66±6%, respectively. Cell membrane integrity was not compromised under the treatment of test Ag species by using a LIVE/DEAD Baclight™ bacterial viability assay. However, electron micrographs demonstrated that Ag NPs attached to the microbial cells, probably causing cell wall pitting. The results suggest that nitrifying bacteria are especially susceptible to inhibition by Ag NPs, and the accumulation of Ag NPs could have detrimental effects on the microorganisms in wastewater treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 42, Issue 12, June 2008, Pages 3066–3074
نویسندگان
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