کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4486471 1316990 2007 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Towards measuring particle-associated fecal indicator bacteria in tropical streams
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فرآیندهای سطح زمین
پیش نمایش صفحه اول مقاله
Towards measuring particle-associated fecal indicator bacteria in tropical streams
چکیده انگلیسی

A protocol to enumerate particle-associated microbial indicator bacteria (heterotrophic plate count [HPC], enterococci [ENT] and Clostridium perfringens [CP]) by membrane filtration in a tropical stream is proposed that relies on high-speed homogenization and chemical treatment. Application of this protocol to stream samples suggest that ENT measurements are more biased by the presence of aggregates than HPC or CP. Whole sample treatment typically increased the colony forming units (CFU) count by 9–52%. Analysis of different settled fractions and examination of the number of indicators recoverable from particles retained on a 5 μm filter in relation to the number of particles containing target indicators both indicate that relatively more ENT form aggregates than HPC or CP. Although the bias is smallest for CP, this does not imply that CP is a better indicator as this depends on the unknown extent to which pathogens are themselves found associated with particles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 41, Issue 7, April 2007, Pages 1501–1515
نویسندگان
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