Segregation analysis indicates that Puroindoline b-2 variants 2 and 3 are allelic in Triticum aestivum and that a revision to Puroindoline b-2 gene symbolization is indicated

► Segregation analysis indicates that Pinb-2v2 and Pinb-2v3 are allelic in T. aestivum. ► Pinb-2 variants 2 and 3 have no effect on hardness in T. aestivum. ► Pinb-2 variants 2 and 3 should be designated Pinb-B2a and Pinb-B2b, respectively. ► Pinb-2 variants 1 and 4 should be designated Pinb-D2a and Pinb-A2a, respectively.