The gene expression profile of Monochamus alternatus in response to deltamethrin exposure

• We created DGE libraries of exposure to deltamethrin.
• The total number of clean reads was 7.139 million in a library of M. alternatus.
• 4886 and 2083 unigenes were significantly up- or down-regulated, respectively.
• The functions of DGEs were annotated by GO and KEGG pathway enrichment analysis.
• The profile of the secondary targets of deltamethrin in M. alternatus is proposed.

To identify additional genes that are potentially associated with deltamethrin toxicology, differentially expressed gene (DGE) libraries of Monochamus alternatus after short-term exposure to a sub-lethal concentration of deltamethrin were prepared. The transcripts were sequenced using the Illumina sequencing platform. After cleaning and quality checks, the total numbers of clean reads were 7.066 and 7.139 million in the control and deltamethrin-exposed libraries, respectively. We found that 4886 and 2083 unique genes were significantly up- and down-regulated, respectively. The gene ontology enrichment analysis identified that 4032, 3379 and 8323 DEGs were involved in cellular components, molecular functions and biological processes, respectively, and showed that the genes were distributed among more than 50 categories. The Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that the top 20 enriched pathways included the glutathione metabolism pathway and the antigen processing and presentation pathway. There were 42 DEGs enriched in the antigen processing and presentation pathway, including those encoding the 70-kDa heat shock protein, protein disulfide isomerase and calreticulin, all of which were up-regulated. There were 61 DEGs enriched in the glutathione metabolism pathway, and these included the up-regulated DEGs encoding glutamate–cysteine ligase, glutathione synthase and glutathione reductase and the down-regulated DEGs encoding glutathione S-transferase, glutathione peroxidase and ornithine decarboxylase. This study is the first step toward achieving an understanding of the profile of the secondary targets of deltamethrin in M. alternatus, and the results may provide insights to further explore the functions of the target genes in detoxification and resistance to deltamethrin.

Figure optionsDownload as PowerPoint slide