کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4550676 | 1627571 | 2015 | 14 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Short-term effects on antioxidant enzymes and long-term genotoxic and carcinogenic potential of CuO nanoparticles compared to bulk CuO and ionic copper in mussels Mytilus galloprovincialis Short-term effects on antioxidant enzymes and long-term genotoxic and carcinogenic potential of CuO nanoparticles compared to bulk CuO and ionic copper in mussels Mytilus galloprovincialis](/preview/png/4550676.png)
• Copper accumulation occurred in mussels exposed to CuO NPs and ionic copper for 21 d.
• CuO NPs, bulk CuO and ionic copper produced different effects on antioxidant activities.
• Micronuclei frequency increased significantly in mussels exposed to CuO NPs.
• Transcription levels of cancer-related genes did not change after exposure to CuO NPs.
• Further studies are needed to determine genotoxic and carcinogenic potential of CuO NPs.
The aim of this work was to study short-term effects on antioxidant enzyme activities and long-term genotoxic and carcinogenic potential of CuO nanoparticles (NPs) in comparison to bulk CuO and ionic copper in mussels Mytilus galloprovincialis after 21 days exposure to 10 μg Cu L−1. Then, mussels were kept for up to 122 days in clean water. Cu accumulation depended on the form of the metal and on the exposure time. CuO NPs were localized in lysosomes of digestive cells, as confirmed by TEM and X ray microanalysis. CuO NPs, bulk CuO and ionic copper produced different effects on antioxidant enzyme activities in digestive glands, overall increasing antioxidant activities. CuO NPs significantly induced catalase and superoxide dismutase activities. Fewer effects were observed in gills. Micronuclei frequency increased significantly in mussels exposed to CuO NPs and one organism treated with CuO NPs showed disseminated neoplasia. However, transcription levels of cancer-related genes did not vary significantly. Thus, short-term exposure to CuO NPs provoked oxidative stress and genotoxicity, but further studies are needed to determine whether these early events can lead to cancer development in mussels.
Journal: Marine Environmental Research - Volume 111, October 2015, Pages 107–120