کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4554424 | 1628073 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Co-expression of ApGSMT and ApDMT promotes biosynthesis of glycine betaine in rice (Oryza sativa L.) and enhances salt and cold tolerance
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Co-expression of ApGSMT and ApDMT promotes biosynthesis of glycine betaine in rice (Oryza sativa L.) and enhances salt and cold tolerance Co-expression of ApGSMT and ApDMT promotes biosynthesis of glycine betaine in rice (Oryza sativa L.) and enhances salt and cold tolerance](/preview/png/4554424.png)
چکیده انگلیسی
Glycine betaine (GB) is an osmoprotectant that protects plants from abiotic stress. GB is biosynthesized by choline dehydrogenation/oxidation and glycine methylation. The latter pathway involves glycine as a substrate and produces GB via successive methylation of glycine by two different N-methyltransferase enzymes; glycine sarcosine methyltransferase (GSMT), and sarcosine dimethylglycine methyltransferase (SDMT). In this study, Agrobacterium-mediated gene transformation was used to produce transgenic rice (Oryza sativa L., cv. Nipponbare) plants containing ApGSMT and ApDMT genes isolated from Aphanothece halophytica. The co-expression of both ApGSMT and ApDMT transgenes resulted in a significant increase of GB biosynthesis and enhanced tolerance to salt and cold stresses in the transgenic rice plants. These results demonstrate the potential of bioengineering for glycine N-methyltransferase genes in crop plants tolerance to abiotic stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 104, August 2014, Pages 16-25
Journal: Environmental and Experimental Botany - Volume 104, August 2014, Pages 16-25
نویسندگان
Xiangli Niu, Fangjie Xiong, Jia Liu, Yuan Sui, Zhengming Zeng, Bao-Rong Lu, Yongsheng Liu,