Impacts of sulfur regulation in vivo on arsenic accumulation and tolerance of hyperaccumulator Pteris vittata

► Sulfur assimilation inhibitor suppressed arsenate reduction. ► Arsenate reduction contributed to arsenic uptake by Pteris vittata. ► Sulfur assimilation accelerant increased As-SH abundance. ► Chelation of arsenic and thiols aided Pteris vittata arsenic tolerance.