On the mechanism of salt tolerance in olive (Olea europaea L.) under low- or high-Ca2+ supply

The effect of changes in Ca2+/Na+ ratios at the root zone has been reported in Olea europaea, a species mostly cultivated in calcareous soils. Plants were exposed to low (2.0 mM, low-Ca) or high-Ca2+ supply (9.0 mM, high-Ca) and supplied with 0 or 200 mM NaCl. Measurements were performed on water relations, gas exchange and photosynthetic performances, ion fluxes at whole-plant and leaf level, Na+ allocation at organismal level, the elemental and soluble carbohydrate concentration in the leaf. Most parameters were also measured during a period of relief from salinity stress, as Olea europaea suffers from fluctuating root zone NaCl concentrations over the whole growing season. High-Ca2+ supply decreased stomatal conductance, especially during the first two weeks of treatment. In response to salinity stress (i) leaf turgor potential was more severely depressed in high-Ca than in low-Ca plants, whereas net CO2 assimilation rate and relative growth rate were unaffected by root zone Ca2+ concentrations (ii) high-Ca plants had a markedly superior ability to both exclude Na+ from the shoot and to selectively transport K+ over Na+ than low-Ca plants; (iii) both CO2 carboxylation efficiency and maximal efficiency of PSII photochemistry (Fv/Fm) were significantly smaller in low-Ca than in high-Ca plants, likely as a result of a greater accumulation of toxic ions. Consistently, when osmotic stress was relieved by supplying plants with good quality water (relief period), both photosynthetic (+44%) and growth rates (+65%) recovered to a markedly superior degree in high-Ca than in low-Ca plants which had been previously treated with 200 mM NaCl. We conclude that (1) high-Ca2+ supply expose olive leaves to a more severe dehydration, but allowed to restrict both the entry and the allocation of potentially toxic ions to sensitive shoot organs; (2) a transient restriction of water-mass flow to the shoot during salinization may be of relatively minor significance in Olea europaea, which is very tolerant to drought; (3) overall salt tolerance in Olea europaea, as in most evergreen sclerophylls inhabiting Mediterranean areas, tightly depends upon the ability to reduce water uptake and transpiration during the dry/warm period and to recover photosynthetic and growth rates when low-salinity flood water is available. Therefore, data from the present experiment allow conclude that an increase in root zone Ca2+ concentration enhances tolerance to salinity stress in olive plants.