کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4555749 | 1628111 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunolocalization of a defense-related 87Â kDa cystatin in leaf blade of tomato plants
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunolocalization of a defense-related 87Â kDa cystatin in leaf blade of tomato plants Immunolocalization of a defense-related 87Â kDa cystatin in leaf blade of tomato plants](/preview/png/4555749.png)
چکیده انگلیسی
In our previous work, we described the defensive potential of a wound- and methyl jasmonate-inducible 87Â kDa tomato cystatin and its accumulation in a crystalline form. Here, we report the immunolocalization of this cysteine proteinase inhibitor in tomato leaf blade. Methyl jasmonate treated wild type plants showed accumulation of crystalline structures that were specifically and strongly stained with polyclonal antibodies against tomato cystatin. Crystalline cystatin was found in palisade and spongy parenchyma cells and immuno-gold electron microscopy analysis revealed that the crystals were compartmentalized in the cytosol. The same pattern in localization of cystatin was observed in transgenic tomato plants superexpressing prosystemin transgene. Our data showing the accumulation of cystatin in response to methyl jasmonate and in response to a overproduction of a wound signal corroborate the protective role of this inhibitor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 55, Issues 1â2, January 2006, Pages 201-208
Journal: Environmental and Experimental Botany - Volume 55, Issues 1â2, January 2006, Pages 201-208
نویسندگان
Hérika C. Madureira, Maura Da Cunha, Tânia Jacinto,