کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4559389 1330439 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid species identification of fresh and processed scallops by multiplex PCR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Rapid species identification of fresh and processed scallops by multiplex PCR
چکیده انگلیسی

Food control policies regarding to seafood label authenticity have become a global issue due to increased incidence of species substitution or mislabelling. Proper species-level identification in processed scallop products is hindered by the lack of morphological characters such as their valves. In order to identify four commercially important scallop species (Argopecten purpuratus, Argopecten irradians, Mizuhopecten yessoensis, Pecten albicans) a species-specific multiplex PCR reaction is described herein. Novel reverse species-specific primers in combination with one universal forward primer designed to amplify a partial region of the mitochondrial 16S rRNA gene were assayed in fresh as well as in manufactured scallop samples. All PCR reactions showed a high specificity allowing an unambiguous species authentication.


► We developed novel primers for the identification of four scallop species.
► All primers showed high specificity and performance.
► Two scallop products from different species were labelled by the same common name.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 32, Issue 2, August 2013, Pages 472–476
نویسندگان
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