Evaluation of the factors affecting the activity of sakacin C2 against E. coli in milk

► Milk fat decreased the activity of sakacin C2 against Escherichia coli in milk. ► Emulsifiers influenced the effect of sakacin C2 against E. coli in milk. ► Nisin and ε-Polylysine increased the activity of sakacin C2 against E. coli in milk. ► Homogenization interfered with the activity of sakacin C2 against E. coli in milk.