کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4559894 1330480 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Pressurized liquid extraction followed by liquid chromatography–mass spectrometry for determination of zearalenone in cereal flours
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Pressurized liquid extraction followed by liquid chromatography–mass spectrometry for determination of zearalenone in cereal flours
چکیده انگلیسی

A method for determination of zearalenone in cereal flour has been developed applying pressurized liquid extraction (PLE) using methanol/acetonitrile (50:50 v/v) as the extraction solvent. The extracted samples were analyzed with liquid chromatography coupled to mass spectrometry (LC–MS) with an electro spray ionisation interface (ESI). The method was validated as a quantitative confirmatory method according to the Eu Commission Decision 2002/657/EC. Recoveries of the extraction step data were satisfactory with values higher then 70%. Quantification limits (LOQ) were 5 μg/kg for ESI (+) and 1 μg/kg for ESI (−). Twenty one flour samples produced in different countries were extracted and analyzed by LC–MS (ESI) negative ionisation followed by LC–MS (ESI) positive ionisation for confirmative purposes, quantification was based on matrix-matched standard curves to compensate disturbing matrix effect. Only one sample was confirmed containing zearalenone below levels regulated by European Commission.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Control - Volume 21, Issue 4, April 2010, Pages 399–402
نویسندگان
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