Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: 2—Behaviour of pathogenic and spoilage bacteria in dual species biofilms including a bacteriocin-like-producing lacti

The survival of Listeria innocua ATCC 33090, Staphylococcus aureus E1S-5 and/or Hafnia alvei E1E-25 in dual species biofilms with bacteriocin-like producing lactic acid bacteria (5 Vagococcus carniphilus, 3 Enterococcus faecium, 1 Lactobacillus sakei and 1 Enterococcus sp.) was investigated. The aim was to select strains able to repress the growth of undesirable bacteria in biofilms, i.e., the real mode of bacterial attachment.Two E. faecium and 3 V. carniphilus species were highly antagonistic to L. innocua, S. aureus and H. alvei repressing their growth by reduction levels able to reach 2, 2.7 and 2.4 log10 cfu/ml compared to the positive control made of sole the target microorganism. Furthermore, planktonic cells were more sensitive to the bacteriocin-like substances than sessile ones.First results suggest the possibility of selecting bio-preservatives among the endogenous house flora of the studied small-scale facility, that could be implemented on the processing surfaces to repress the growth of undesirable microorganisms.