کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4563529 1628526 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Soy protein hydrolysates fermentation: Effect of debittering and degradation of major soy allergens
ترجمه فارسی عنوان
تخمیر هیدرولیزهای پروتئین سویا: اثر تخلیه و تخریب آلرژن های بزرگ سویا
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
چکیده انگلیسی


• Effect of fermentation on reduction of bitter taste of soy protein hydrolysates was studied.
• Major soy allergens (Gly m5 and Gly m6) were effectively degraded.
• Bitterness of hydrolysates was significantly (p < 0.05) decreased.
• Superior techno-functional properties, i.e. emulsifying capacity, were achieved.

The debittering effect of induced liquid state fermentation (Lactobacillus perolens, Rhizopus oryzae, and Actinomucor elegans) on different soy protein hydrolysates has been investigated. The hydrolytic action was monitored by SDS-PAGE and degree of hydrolysis analyses. Sensory perception using quantitative descriptive analysis (QDA), employing multivariate statistical principal component analysis (PCA), techno-functional properties, and the microbial competitiveness (MALDI-TOF-MS) have been evaluated. SDS-PAGE profiles evidenced that the enzyme preparations degraded most of major soy allergens (β-conglycinin, glycinin), while subsequent fermentation did not further change the profiles. All strains investigated effectively reduced bitterness to a minimum of 0.7 on a 10-cm continuous scale (0 = no perception; 10 = strong perception) compared to non-fermented hydrolysates (2.8–8.0) and untreated soy protein isolate (2.8). Protein solubility, emulsifying and oil-binding capacity as well as foaming activity and gelation behaviour were enhanced depending on the protease used; subsequent fermentation further improved foaming stability and gelation concentration. PCA of descriptive sensory data revealed that fermentation apparently upgrade the organoleptic perception by effectively decreasing the bitter taste, simultaneously reducing the beany off-flavour of soy. Consequently, enzymatic hydrolysis combined with subsequent fermentation represents a promising method for the production of hypoallergenic soy hydrolysates with pleasant taste and great technofunctionality.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: LWT - Food Science and Technology - Volume 71, September 2016, Pages 202–212
نویسندگان
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