کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4760755 | 1422426 | 2016 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a discordant genotype at the D2S1338 locus between Identifiler Plus®16 kit and Power Plex®21 system kit
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Genotyping errors can perturb estimates of genetic diversity and adversely affects the reliability of forensic identification, which has become increasingly recognized. During our daily laboratory work, we hit upon a discordant short tandem repeat (STR) genotype at the D2S1338 locus between Identifiler Plus®16 system kit and Power Plex®21 system kit. To figure out the reason of the discordance, nested PCR was performed to amplify the D2S1338 locus and then the final amplicons were sequenced. We found a G to G/A transition at the position on which caused an allele dropout of Identifiler Plus®16 kit.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Forensic and Legal Medicine - Volume 44, November 2016, Pages 174-177
Journal: Journal of Forensic and Legal Medicine - Volume 44, November 2016, Pages 174-177
نویسندگان
Lin Zhang, Hongyan Wu, Kejie Wang, Aiying Fan, Jiafeng Niu, Zhendong Zhu,