کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4908303 | 1426596 | 2016 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Loading of silicon nanoparticle labels with redox mediators for detection of multiple DNA targets within a single voltammetric sweep
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
We have constructed electrochemical labels by loading four different redox mediators into silica nanoparticles (SiNPs) through electrostatic attraction. The mediators were methylene blue, ferrocinium tetrafluoroborate, tris (2,2-bipyridyl) dichlororuthenium (II) hexahydrate and acridine orange. The SiNPs were coated with avidin, which enabled attachment to biotin-tagged DNA sequences, forming signal probes. The immobilisation of four different capture probes onto screen printed electrodes enabled a simple qualitative and semi-quantitative detection of four different target probes within a single voltammetric sweep using differential pulse voltammetry. This was illustrated by detecting sequences found in four forms of the influenza virus, the influenza A sub-types H1N1, H3N2, H5N1 and influenza B. The limits of detection were 1.6Â pM, 1.6Â pM, 4.7Â pM and 2.9Â pM, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 779, 15 October 2016, Pages 61-66
Journal: Journal of Electroanalytical Chemistry - Volume 779, 15 October 2016, Pages 61-66
نویسندگان
Nopchulee Cheeveewattanagul, Patsamon Rijiravanich, Werasak Surareungchai, Mithran Somasundrum,