کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4908303 1426596 2016 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Loading of silicon nanoparticle labels with redox mediators for detection of multiple DNA targets within a single voltammetric sweep
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Loading of silicon nanoparticle labels with redox mediators for detection of multiple DNA targets within a single voltammetric sweep
چکیده انگلیسی
We have constructed electrochemical labels by loading four different redox mediators into silica nanoparticles (SiNPs) through electrostatic attraction. The mediators were methylene blue, ferrocinium tetrafluoroborate, tris (2,2-bipyridyl) dichlororuthenium (II) hexahydrate and acridine orange. The SiNPs were coated with avidin, which enabled attachment to biotin-tagged DNA sequences, forming signal probes. The immobilisation of four different capture probes onto screen printed electrodes enabled a simple qualitative and semi-quantitative detection of four different target probes within a single voltammetric sweep using differential pulse voltammetry. This was illustrated by detecting sequences found in four forms of the influenza virus, the influenza A sub-types H1N1, H3N2, H5N1 and influenza B. The limits of detection were 1.6 pM, 1.6 pM, 4.7 pM and 2.9 pM, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 779, 15 October 2016, Pages 61-66
نویسندگان
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