کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5028 | 271 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles Refolding of denatured lysozyme assisted by artificial chaperones in reverse micelles](/preview/png/5028.png)
چکیده انگلیسی
This article reports oxidative protein refolding assisted by artificial chaperones in reverse micelles formed by nonionic surfactant of sorbitan trioleate modified with Cibacron Blue F-3GA in n-hexane. The denatured/reduced lysozyme was used as a model protein and cetyltrimethylammonium bromide (CTAB) and β-cyclodextrin as the artificial chaperones. The use of the artificial chaperones has proved to increase the refolding efficiency of denatured–reduced lysozyme at the concentration range studied (3.5–5.9 mg/mL). Moreover, the artificial chaperones increased the refolding yield in a wide range of urea concentrations. However, the optimal urea concentration range was little affected by the presence of the artificial chaperones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 92–95
Journal: Biochemical Engineering Journal - Volume 31, Issue 1, 1 August 2006, Pages 92–95
نویسندگان
Xiao-Yan Dong, Xiao-Yue Wu, Yan Sun,