کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5040634 1473903 2017 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effects of dexmedetomidine pretreatment on the pro- and anti-inflammation systems after spinal cord injury in rats
ترجمه فارسی عنوان
اثرات پیشگیری از تزریق دکستومیدینید بر سیستم های پروتئین و ضد التهابی پس از آسیب نخاعی در موش صحرایی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


- SCI caused a significant increase in the expression of TLR4 and α7nAChR.
- DEX prevented altered expression of TLR4, but significantly amplified expression of TLR4 in α7nAChR.
- DEX-induced downregulation of TLR4, at least in part, required activation of the α7nAChR/PI3K/Akt/PU.1 pathway.
- DEX-induced upregulation of α7nAChR required activation of the α2R/PI3K/Akt/Ly6h pathway.
- Both TLR4 and α7nAChR were primarily expressed in neurons during the early phase of SCI.

Excessive inflammatory responses play important roles in the aggravation of secondary damage to an injured spinal cord. Dexmedetomidine (DEX), a selective α2-adrenoceptor agonist, has recently been implied to be neuroprotective in clinical anesthesia, but the underlying mechanism is elusive. As signaling through Toll-like receptor 4 (TLR4) and nicotinic receptors (nAChRs, notably α7nAChR) play important roles in the pro- and anti-inflammation systems in the central nervous system, respectively, this study investigated whether and how they were modulated by DEX pretreatment in a rat model of spinal cord compression. The model was used to mimic perioperative compressive spinal cord injury (SCI) during spinal correction. DEX preconditioning improved locomotor scores after SCI, which was accompanied by increased α7nAChR and acetylcholine (Ach, an endogenous ligand of α7nAChR) expression as well as PI3K/Akt activation. However, there was a decrease in Ly6h (a negative regulator for α7nAChR trafficking), TLR4, PU.1 (a critical transcriptional regulator of TLR4), HMGB1 (an endogenous ligand of TLR4), and caspase 3-positive cells, which was prevented by intrathecal preconditioning with antagonists of either α2R, α7nAChR or PI3K/Akt. In addition, application of an α7nAChR agonist produced effects similar to those of DEX after SCI, while application of an α7nAChR antagonist reversed these effects. Furthermore, both α7nAChR and TLR4 were mainly co-expressed in NeuN-positive cells of the spinal ventral horn, but not in microglia or astrocytes after SCI. These findings imply that the α2R/PI3K/Akt/Ly6h and α7nAChR/PI3K/Akt/PU.1 cascades are required for upregulated α7nAChR and downregulated TLR4 expression by DEX pretreatment, respectively, which provided a unique insight into understanding DEX-mediated neuroprotection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain, Behavior, and Immunity - Volume 64, August 2017, Pages 195-207
نویسندگان
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