کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5130899 1490866 2017 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Review: Microbial analysis in dielectrophoretic microfluidic systems
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Review: Microbial analysis in dielectrophoretic microfluidic systems
چکیده انگلیسی


- Microfluidic electrokinetic manipulation of microbials to enhance detection sensitivity.
- Characteristic cell shape and subcellular electrophysiology enables selective polarization.
- Dielectrophoretic manipulation platforms for selective microbial capture on sensor.
- Platforms for coupling selective microbial enrichment to their detection.
- Probing subcellular phenotypic microbial alterations to monitor antibiotic susceptibility.

Infections caused by various known and emerging pathogenic microorganisms, including antibiotic-resistant strains, are a major threat to global health and well-being. This highlights the urgent need for detection systems for microbial identification, quantification and characterization towards assessing infections, prescribing therapies and understanding the dynamic cellular modifications. Current state-of-the-art microbial detection systems exhibit a trade-off between sensitivity and assay time, which could be alleviated by selective and label-free microbial capture onto the sensor surface from dilute samples. AC electrokinetic methods, such as dielectrophoresis, enable frequency-selective capture of viable microbial cells and spores due to polarization based on their distinguishing size, shape and sub-cellular compositional characteristics, for downstream coupling to various detection modalities. Following elucidation of the polarization mechanisms that distinguish bacterial cells from each other, as well as from mammalian cells, this review compares the microfluidic platforms for dielectrophoretic manipulation of microbials and their coupling to various detection modalities, including immuno-capture, impedance measurement, Raman spectroscopy and nucleic acid amplification methods, as well as for phenotypic assessment of microbial viability and antibiotic susceptibility. Based on the urgent need within point-of-care diagnostics towards reducing assay times and enhancing capture of the target organism, as well as the emerging interest in isolating intact microbials based on their phenotype and subcellular features, we envision widespread adoption of these label-free and selective electrokinetic techniques.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytica Chimica Acta - Volume 966, 8 May 2017, Pages 11-33
نویسندگان
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